RESUMO
The isolation frequency of Arcobacter species in children with diarrhea, fowls, mammals and food of avian and marine origin was established. In all the samples it was possible to isolate Arcobacter species corresponding 201 (39.4%) to A. butzleri and 24 (4.7) to A. cryaerophilus . Both species were simultaneously isolated in 19 (3.7%) being A. butzleri the most frequently isolated species.
Assuntos
Arcobacter/isolamento & purificação , Microbiologia de Alimentos , Infecções por Bactérias Gram-Negativas/microbiologia , Infecções por Bactérias Gram-Negativas/veterinária , Animais , Aves , Criança , Chile/epidemiologia , Coinfecção/epidemiologia , Coinfecção/microbiologia , Coinfecção/veterinária , Diarreia/epidemiologia , Diarreia/microbiologia , Diarreia/veterinária , Reservatórios de Doenças , Infecções por Bactérias Gram-Negativas/epidemiologia , Humanos , Mamíferos , PrevalênciaRESUMO
The isolation frequency of Arcobacter species in children with diarrhea, fowls, mammals and food of avian and marine origin was established. In all the samples it was possible to isolate Arcobacter species corresponding 201 (39.4%) to A. butzleri and 24 (4.7) to A. cryaerophilus. Both species were simultaneously isolated in 19 (3.7%) being A. butzleri the most frequently isolated species.
Assuntos
Animais , Criança , Humanos , Arcobacter/isolamento & purificação , Microbiologia de Alimentos , Infecções por Bactérias Gram-Negativas/microbiologia , Infecções por Bactérias Gram-Negativas/veterinária , Aves , Chile/epidemiologia , Coinfecção/epidemiologia , Coinfecção/microbiologia , Coinfecção/veterinária , Reservatórios de Doenças , Diarreia/epidemiologia , Diarreia/microbiologia , Diarreia/veterinária , Infecções por Bactérias Gram-Negativas/epidemiologia , Mamíferos , PrevalênciaRESUMO
Edwarsiella tarda is a zoonotic bacterium that can be isolated from humans, animals and the environment. Although E. tarda is primarily considered a fish pathogen, it is the only species of its genus considered to be pathogenic for humans as well. A survey of zoonotic intestinal bacteria in fresh feces from South American sea lions (SASL) Otaria flavescens, reported E. tarda as the most frequently isolated species. In this study, we used HEp-2 cells to establish in vitro the adherence and invasive ability of 17 E. tarda strains isolated from SASL fecal material. All the strains were able to adhere and invade HEp-2 cells with adhesion and invasion percentages ranging from 56 to 100% and 21 to 74%, respectively. Despite the expression of these pathogenic factors, further investigation is needed to determine whether this bacterium could play a role as primary pathogen for this and other species of pinnipeds.
Assuntos
Aderência Bacteriana , Edwardsiella tarda/fisiologia , Endocitose , Infecções por Enterobacteriaceae/veterinária , Hepatócitos/microbiologia , Leões-Marinhos/microbiologia , Animais , Edwardsiella tarda/isolamento & purificação , Infecções por Enterobacteriaceae/microbiologia , Células Hep G2 , Humanos , América do SulRESUMO
Edwarsiella tarda is a zoonotic bacterium that can be isolated from humans, animals and the environment. Although E. tarda is primarily considered a fish pathogen, it is the only species of its genus considered to be pathogenic for humans as well. A survey of zoonotic intestinal bacteria in fresh feces from South American sea lions (SASL) Otaria flavescens, reported E. tarda as the most frequently isolated species. In this study, we used HEp-2 cells to establish in vitro the adherence and invasive ability of 17 E. tarda strains isolated from SASL fecal material. All the strains were able to adhere and invade HEp-2 cells with adhesion and invasion percentages ranging from 56 to 100% and 21 to 74%, respectively. Despite the expression of these pathogenic factors, further investigation is needed to determine whether this bacterium could play a role as primary pathogen for this and other species of pinnipeds.
